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parp1 fragments  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc parp1 fragments
    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
    Parp1 Fragments, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 15328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/parp1+fragments/PARP+Antibody/pm33867143-97-12-18
    Average 98 stars, based on 15328 article reviews
    parp1 fragments - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer."

    Article Title: Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer.

    Journal: Gynecologic oncology

    doi: 10.1016/j.ygyno.2021.04.015

    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of PARP1. [Con – Control; Ola – Olaparib; Ent – Entinostat].
    Figure Legend Snippet: Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of PARP1. [Con – Control; Ola – Olaparib; Ent – Entinostat].

    Techniques Used: Expressing, Staining, Comparison, Western Blot, Sulforhodamine B Assay, Marker, Control

    Fig. 6. Graphical summary indicating the mechanism for entinostat-induced olaparib sensitivity in HR-proficient cells. As described, entinostat causes breakdown of HR-DNA repair pathway, whereas olaparib inactivates PARP, crippling the cell's potential to repair SSBs. This in turn leads to accumulation of DSBs, which the cell is unable to repair due to defective HR repair pathway, accumulating unrepaired DNA ultimately resulting in cell death.
    Figure Legend Snippet: Fig. 6. Graphical summary indicating the mechanism for entinostat-induced olaparib sensitivity in HR-proficient cells. As described, entinostat causes breakdown of HR-DNA repair pathway, whereas olaparib inactivates PARP, crippling the cell's potential to repair SSBs. This in turn leads to accumulation of DSBs, which the cell is unable to repair due to defective HR repair pathway, accumulating unrepaired DNA ultimately resulting in cell death.

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer.
    Article Snippet: .. The primary antibody was a rabbit polyclonal against cleaved PARP that detects PARP1 fragments, but not intact PARP1 (Cell Signaling Technology; 5625; 1:100) for overnight incubation at 4 °C and amousemonoclonal against β-actin (Life Technologies; MA1–140) was used as loading control. ..

    Control:

    Article Title: Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer.
    Article Snippet: .. The primary antibody was a rabbit polyclonal against cleaved PARP that detects PARP1 fragments, but not intact PARP1 (Cell Signaling Technology; 5625; 1:100) for overnight incubation at 4 °C and amousemonoclonal against β-actin (Life Technologies; MA1–140) was used as loading control. ..



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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of <t>PARP1.</t> [Con – Control; Ola – Olaparib; Ent – Entinostat].
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    Image Search Results


    Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of PARP1. [Con – Control; Ola – Olaparib; Ent – Entinostat].

    Journal: Gynecologic oncology

    Article Title: Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer.

    doi: 10.1016/j.ygyno.2021.04.015

    Figure Lengend Snippet: Fig. 4. Olaparib combined with entinostat decreases proliferation, alters HR machinery and increases apoptosis in HR-proficient SKOV-3 cells. (A). Immunofluorescence showing that Ola + Ent significantly reduces BRCA1 and Ki-67 expression levels in SKOV-3 cells. DAPI was used as nuclear stain (40 × 100 μm scale bar). Quantification of BRCA1 and Ki-67 indicated significant downregulation in cells treated with Ola + Ent (*p < 0.05, **p < 0.005 Students t-test). (B) BRCA1 transcript levels showed significant downregulation in all treatment groups, with enhanced downregulation in Ola + Ent (*p < 0.012; **p < 0.0036; ***p < 0.0005, one-way ANOVA; Sidak's multiple comparison test) indicating that BRCA1 expression is affected markedly at the mRNA levels compared to protein levels. (C) Western blot analysis of SKOV-3 cells treated with Ola + Ent as described for SRB assay showing increased cleaved PARP expression in olaparib and Ola + Ent treated cells. Quantification showed significant upregulation of cleaved PARP, a marker of cell death (*p < 0.05; **p < 0.005, Students t-test). The antibody used was specific for fragments of PARP1. [Con – Control; Ola – Olaparib; Ent – Entinostat].

    Article Snippet: The primary antibody was a rabbit polyclonal against cleaved PARP that detects PARP1 fragments, but not intact PARP1 (Cell Signaling Technology; 5625; 1:100) for overnight incubation at 4 °C and amousemonoclonal against β-actin (Life Technologies; MA1–140) was used as loading control.

    Techniques: Expressing, Staining, Comparison, Western Blot, Sulforhodamine B Assay, Marker, Control

    Fig. 6. Graphical summary indicating the mechanism for entinostat-induced olaparib sensitivity in HR-proficient cells. As described, entinostat causes breakdown of HR-DNA repair pathway, whereas olaparib inactivates PARP, crippling the cell's potential to repair SSBs. This in turn leads to accumulation of DSBs, which the cell is unable to repair due to defective HR repair pathway, accumulating unrepaired DNA ultimately resulting in cell death.

    Journal: Gynecologic oncology

    Article Title: Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer.

    doi: 10.1016/j.ygyno.2021.04.015

    Figure Lengend Snippet: Fig. 6. Graphical summary indicating the mechanism for entinostat-induced olaparib sensitivity in HR-proficient cells. As described, entinostat causes breakdown of HR-DNA repair pathway, whereas olaparib inactivates PARP, crippling the cell's potential to repair SSBs. This in turn leads to accumulation of DSBs, which the cell is unable to repair due to defective HR repair pathway, accumulating unrepaired DNA ultimately resulting in cell death.

    Article Snippet: The primary antibody was a rabbit polyclonal against cleaved PARP that detects PARP1 fragments, but not intact PARP1 (Cell Signaling Technology; 5625; 1:100) for overnight incubation at 4 °C and amousemonoclonal against β-actin (Life Technologies; MA1–140) was used as loading control.

    Techniques: